Part:BBa_K2680119:Design

3G CI-repressible promoter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 41
  Illegal BsaI.rc site found at 106
  

Design Notes

Sequence from Murray Lab at California Institute of Technology. Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.

Source

"Based on the pR promoter from bacteriophage lambda. The specific sequence used here [in BBa_R0051] is based on the cI repressible promoter used in the Elowitz repressilator (and references therein)."¹

"In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction [...] Incompatible with host expressing cI repressor."²

References

¹Mahajan, V. S., Marinescu, V. D., Chow, B., Wissner-Gross, A. D., & Carr, P. (2003, January 31). Part:BBa_R0051. Retrieved October 15, 2018, from https://parts.igem.org/Part:BBa_R0051

²Mahajan, V. S., Marinescu, V. D., Chow, B., Wissner-Gross, A. D., & Carr, P. (2003, January 31). Part:BBa_R0051:Design. Retrieved October 15, 2018, from https://parts.igem.org/Part:BBa_R0051:Design

Elowitz repressilator paper: Elowitz, M. B., & Leibler, S. (2000). A synthetic oscillatory network of transcriptional regulators. Nature, 403(6767), 335-338. doi:10.1038/35002125